TRICHODERMA HARZIANUM SELECTIVE AGAR BASE

Trichoderma harzianum is an efficient biocontrol agent that is commercially produced to prevent development of several soil pathogenic fungi. Different mechanisms have been suggested as being responsible for their biocontrol activity, which include competition for space and nutrients, secretion of chitinolytic enzymes, mycoparasitism and production of inhibitory compounds. Nevertheless, the biocontrol activity of T. harzianum could be affected by environmental, that include among others, the presence of plant nutrients at the field level. Also, the isolates of Trichoderma harzianum attack the commercial mushroom Agaricus bisporus colonize spawned compost and result in substantial yield reduction. This medium was based on the formulation of T.harzianum selective medium. Care should be taken not to expose this medium to light, since photodegradation of Rose Bengal yields compound that are toxic to fungi.

for selective isolation of Trichoderma harzianum