STUART TRANSPORT MEDIUM W/O METHYLENE BLUE WITH CHARCOAL

Stuart Transport media were originally designed by Stuart while studying Gonococci. Stuart et al later on modified the Stuart Medium for the transportation of gonococcal specimens for culturing. Ringertz included thioglycollate in the Stuart Medium and omitted charcoal. This medium may be used for the transportation of many fastidious organisms including the anaerobes by maintaining organism’s viability without significant multiplication. Crooks and Stuart suggested the addition of Polymyxin B sulphate which facilitates the recovery of Neisseria gonorrhoeae.
The medium is chemically defined, semisolid, non-nutrient. It prevents microbial proliferation. Composition of the medium ensures that microorganisms present are able to survive for a sufficiently long period of time. The medium provides adequate degree of anaerobiosis. Prepared sterile medium undergoes a slight degree of oxidation at the upper periphery of the medium. Calcium chloride alongwith sodium glycerophosphate act as good buffering agent and also maintains osmotic equilibrium in the medium.

for the preservation and transportation of Neisseria species and other fastidious organisms from the clinic to laboratory