SOYA CASEIN DIGEST AGAR (TRYPTONE SOYA AGAR) (CASO AGAR) (as per USP/EP/JP/BP)

Soybean Casein Digest Agar is a dependable general purpose medium for the growth of a wide variety of microorganisms particularly those from the pharmaceutical products. It is trusted in quality control laboratories and is specified by the major pharmacopoeias like USP, EP, BP, JP and IP for sterility testing and for microbial limit testing. Soybean Casein Digest Agar is also frequently employed in the validation of sterility testing procedures where uniformity and reliability are crucial. The strength of Soybean Casein Digest Agar lies in its nutrient rich composition. It is made from the pancreatic digest of casein and papaic digest of soyabean, which is a source of essential amino acids and peptides required for the growth of microorganisms. These nutrients allow the medium to support both fastidious and non-fastidious bacteria, making it broadly applicable. Natural constituents of the soybean not only aid in promoting growth, but sodium chloride also maintains the osmotic balance necessary for the functioning of the cell. Agar is used as the solidifying agent to provide the appropriate firmness to the medium for observing colonies.

Soybean Casein Digest Agar is commonly used in the microbial limit tests and antimicrobial preservative effectiveness studies. In addition, it is also involved in observation of hemolytic reaction with addition of blood, which is useful in the further analysis of microbes. The aerobic count is the number of colonies that grow on this medium, including any fungal colonies that may appear. Preparation of Soybean Casein Digest Agar is easy to prepare and convenient to use. The medium can be prepared suspension of the powder in water, heating, and sterilizing, or it can be used as a ready to use pre-sterilized medium that only needs melting prior pouring into plates or tubes. After solidification, it gives a clean to slightly opalescent surface for the observation of microbial growth. Soybean Casein Digest Agar is tested through growth promotion studies to verify its consistent performance. It promotes the growth of bacteria within 18 to 24 hours and the growth of fungi in a few days when used under the recommended conditions. In general, Soybean Casein Digest Agar is a reliable medium for day to day microbiological analysis in the pharmaceutical industry.

for the cultivation of various microorganisms from pharmaceuticals products in accordance with harmonized method