LACTIC AGAR

Lactic Agar was developed by Elliker et al and recommended by APHA for cultivation of lactic bacteria to promote the colony development of Lactobacilli and lactic Streptococci. Samples are analyzed by pour plate technique. Lactic acid bacteria are fastidious in nature and hence Lactic Agar is designed to satisfy their growth requirement. Lactic acid bacteria survive at low pH, but are very sensitive to other adverse conditions. Samples to be examined for enumeration of viable lactic acid bacteria should not be frozen prior to analysis. Many of the lactic acid bacteria are easily killed or injured by freezing. For dilution of products it is best to use sterile 0.1% Peptone Water as the diluent since it protects bacteria during the dilution process.

for enumeration and identi?cation of lactic Streptococci and Lactobacilli by pour plate method