L.MONO BLOOD AGAR BASE

L. monocytogenes is a gram positive, facultatively anaerobic rod shaped bacteria. It can grow under refrigerated condition and therefore is a major concern to the food industry. The recovery of Listeria is very low from food and environmental samples, hence it requires enrichment and then further isolation. Various selective and differential media have been proposed for the detection of Listeria species in particular L. monocytogenes.
L.mono Blood Agar Base was developed by Johanson and Kankare for the isolation of Listeria species. It uses Tryptone Soya Agar as a base with the addition of lithium chloride as a selective agent. This medium with the addition of 5% w/v sterile defibrinated sheep blood helps in the differentiation of haemolytic and pathogenic Listeria species which includes L. monocytogenes, L. seeligeri and L. ivanovii from non-haemolytic and non-pathogenic species which include L. innocua, L. grayi and L. welshimeri.

for the speci?c isolation and cultivation of Listeria species from food and environmental samples