ETHYL VIOLET AZIDE BROTH (E.V.A. BROTH) (as per BIS)

Ethyl Violet Azide Broth was formulated according to the recommendations of Litsky, Malmann and Fifield, who investigated the action of a number of dyes and selective agents for the formulation of a confirmation medium for fecal streptococci. BIS has recommended E.V.A Broth for enumeration of Enterococci using MPN technique. The selectivity of the medium for enterococci is due to the presence of ethyl violet and sodium azide, which inhibit the growth of Gram-negative bacilli and sporulated Gram-positive species.

For selective and con?rmatory detection of Enterococci as an indicator of faecal pollution in water.