Product Code TM 369
Deoxycholate Citrate Agar is prepared as per the modified formula of Leifson. This medium is used for the isolation and maximum recovery of intestinal pathogens belonging to Salmonella and Shigella groups from foods. However, it is recommended to use less inhibitory medium when Shigella have to be isolated. The selectivity of this medium permits the use of fairly heavy innocula without danger of overgrowth of Shigella and Salmonella by other microflora. For the routine examination of stool and urine specimens, it is suggested that other media such as MacConkey Agar, Bismuth Sulphite Agar etc. be used in conjunction with this medium. This medium is similar to deoxycholate agar in comparison but is moderately more selective for enteric pathogens owing to increased concentrations of both citrate and deoxycholate salts. Sodium deoxycholate at pH 7.3 to 7.5 is inhibitory for gram positive bacteria. Citrate salts, in the concentration included in the formulation, are inhibitory to gram-positive bacteria and most other normal intestinal organisms.
for isolation of enteric pathogens especially Salmonella and Shigella species
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