Product Code TM 1517
Candidiasis is an acute or sub-acute infection caused by members of the genus Candida, mainly Candida albicans, although all species may be pathogenic. C. albicans produce lesions in the mouth, oesophagus, genitourinary tract, skin, nails, bronchi, lungs and other organs in patients whose normal defense mechanism may have been altered by underlying disease, antimicrobial therapy or immunosuppressive agents. Since saprophytic yeast are microscopically similar to the pathogenic species, all infected material should be cultured on duplicate sets of media with and without antifungal agents.
Candida Agar was developed as described by Fung and Liang, which is a modification of Yeast and Mould Agar. It is a nutritionally rich medium, which supports the growth of many yeasts and moulds and is differential for C. albicans. Goldschmidt demonstrated that YM agar with aniline blue could be used to identify C. albicans with high accuracy and predictability. Aniline blue is a fluorescent indicator, metabolized by C. albicans to produce a fluorescent moiety that can be easily detected under UV light.
Some strains of C. parapsilosis, C. krusei and C. pulcherrima may give slight fluorescence and that may be distinguished from C. albicans by germ tube formation. Specimen is processed and inoculated directly onto the surface of the media.
for isolation and differentiation of Candiffa albicans
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