BURKHOLDERIA CEPACIA SELECTIVE AGAR BASE

Burkholderia cepacia is an important opportunistic pathogen and causes pulmonary infection among individuals with cystic ffibrosis (CF). Burkholderia cepacia species are gram negative, rod shaped bacteria. The organism may lead to Burkholderia cepacia syndrome, a neutralizing pneumonia associated with fever that culminates in to a rapid and fatal clinical deterioration. Burkholderia cepacia species may cause severe infection in individuals with cystic ffibrosis and immunosuppressed individuals. B. cepacia is difficult to isolate on routinely used laboratory media like MacConkey Agar, since B.cepacia is a slow grower and therefore it is usually outgrown by the faster growing Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. Burkholderia Cepacia Agar is based on PC medium, which was originally devised by Gilligan. This medium was found to be superior to MacConkey Agar for growth of B. cepacia. Burkholderia cepacia have the potential of overcoming antimicrobial preservative systems and antiseptics, and can grow in preserved aqueous oral liquids and topical products. This medium is recommended for detection of Burkholderia cepacia in pharmaceutical products. Test procedure: The sample is initially enriched in Soyabean Casein Digest Medium and then plated on Burkholderia Cepacia Selective Agar.

for isolation of Burkholderia cepacia from the respiratory secretions of patients with cystic fibrosis and other non-clinical specimens