BILE PEPTONE TRANSPORT MEDIUM

Vibrio cholerae is the etiological agent of epidemic and pandemic cholera in humans. V. cholerae is a non-halophilic Vibrio which cannot grow in media with a concentration of sodium chloride greater than 5-6% and is able to grow in media lacking NaCl. Human disease is associated with ingestion of contaminated water or food. Vibrio species, like many other gram-negative bacteria, grow in the presence of relatively high levels of bile salts. Bile Peptone Transport medium is a nutritive, selective holding medium for Vibrio species. It is used as a transport (holding) medium to maintain the viability of V. cholerae in stool specimens during delay in transmission to the laboratory.
The sample should be transported immediately to the laboratory. However, if there is to be a delay of more than 6 hours, 1-3 ml of faeces should be added to 10-20 ml of Bile Peptone Transport Medium with tellurite.

for transporting specimens in hot climates prone to Cholera outbreak