ANTIBIOTIC ASSAY MEDIUM NO. 10 (POLYMYXIN SEED AGAR) (PART – I)

Antibiotic Assay media are used in the performance of antibiotic assays. Grove and Randall have elucidated those antibiotic assays and media in their comprehensive treatise on antibiotic assays. Schmidt and Moyer have reported the use of antibiotic assay medium for the liquid formulation used in the performance of antibiotic assay. Freshly prepared plates should be used for antibiotic assays. Test organisms are inoculated in sterile seed agar cooled to 40-45?C and spread evenly over the surface of solidified base agar. After incubation the concentration of the antibiotic being assayed is determined by measuring the zone of inhibition obtained, with that of reference standard antibiotic. All conditions in the microbiological assay must be carefully controlled. The use of standard culture media in the test is one of the important steps for good results.

for assay of Polymyxin-B, Carbenicillin, Colistin and Colistimethate Sodium