ANTIBIOTIC ASSAY MEDIUM G

Antibiotic Assay Medium is formulated in accordance with European and British Pharmacopoeia. This medium is employed widely as base agar for agar diffusion assay of Bleomycin using Mycobacterium smegmatis. Antibiotic Assay Medium is also used for preparing the inoculum of Mycobacterium smegmatis for assay. To perform the antibiotic assay, the Base Agar should be prepared on the same day as the test. For the cylinder method, a base layer of 21 ml is required. Once the base medium has solidified, the seed layer inoculated with the standardized culture can be overlaid. Even distribution of the layer is important. The nutrients essential for growth of test organisms is provided by peptone and meat extract in this medium. Agar provides an excellent solid substratum for support and over layering of seed agar, for the assay of Bleomycin. Addition of glycerol is important for provision of carbon to the test organism.

for the microbiological assay of Bleomycin sulphate using Mycobacterium smegmatis