ANTIBIOTIC ASSAY MEDIUM G

This medium is formulated in accordance to European and British Pharmacopoeia. This medium is employed widely as base agar for agar diffusion assay of Bleomycin using Mycobacterium smegmatis. It is also used for preparing the inoculum of Mycobacterim smegmatis for assay.
To perform the antibiotic assay, the Base Agar should be prepared on the same day as the test. For the cylinder method, a base layer of 21 ml is required. Once the base medium has solidified, seed layer inoculated with the standardized culture can be overlaid. Even distribution of the layer is important.

for the microbiological assay of Bleomycin sulphate using Mycobacterium smegmatis