ANTIBIOTIC ASSAY MEDIUM F (as per IP)

The composition of this medium is in accordance with IP and CFR. This medium is used to prepare the base layer to assay tetracycline and oxytetracycline.
This medium provides the optimal pH 5.8-6.0 for assay of tetracycline as these antibiotics are stable at slightly lower pH. This pH condition also supports the growth of test organisms. To perform the antibiotic assay, the Base Agar should be prepared on the same day as the test. The potency of an antibiotic can be demonstrated by its inhibitory effect on microorganisms under suitable conditions. For the cylinder method, a base layer of 21 ml is required. Once the base medium has solidified, seed layer inoculated with the standardized test culture can be overlaid. Even distribution of the layer is important.

for microbiological assay of Tetracycline & Oxytetracyline