Mueller Hinton Agar (MHA) is a non-selective solid media widely used in microbiology for various applications, with its primary focus on antimicrobial susceptibility testing. In this blog, we will explore the composition, principle, uses, and quality control of MHA, shedding light on its significance in the field of microbiology.
Mueller Hinton Agar is composed of carefully selected ingredients to provide a suitable environment for the growth of a variety of bacteria. The key components and their concentrations are outlined in the table below:
Ingredients | g/l |
Beef infusion | 300 |
Casein acid hydrolysate | 17.500 |
Starch | 1.500 |
Agar | 17.000 |
Principle:
The primary and most crucial application of MHA is in antimicrobial susceptibility testing. It has become the standard medium for the Kirby Bauer method, with its performance specified by the Clinical and Laboratory Standards Institute (CLSI).
Initially developed for the cultivation of pathogenic Neisseria species, MHA is still being used for the cultivation of Neisseria.
MHA is specified in the FDA Bacteriological Analytical Manual for food testing and commonly used procedures on aerobic and facultative anaerobic bacteria.
Non-selective and non-differential:
Starch Content:
Loose Agar:
Batch-to-Batch Reproducibility:
Low in Inhibitors:
To ensure the reliability of MHA in microbiological applications, positive controls can be employed:
By regularly performing quality control measures, laboratories can maintain the effectiveness and consistency of Mueller Hinton Agar for antimicrobial susceptibility testing.
Mueller Hinton Agar remains a cornerstone in microbiology laboratories, especially in antimicrobial susceptibility testing. Its well-balanced composition, coupled with ease of use and reliable performance, makes it an indispensable tool for researchers and healthcare professionals alike. As technology advances, MHA continues to play a crucial role in the accurate determination of bacterial susceptibility to antimicrobial agents, contributing to the ongoing battle against infectious diseases.
At TM Media , we take pride in offering high-quality Mueller Hinton Agar (MHA) and its variants, meticulously designed for precise antimicrobial susceptibility testing and microbial cultivation. Our MHA formulations, including those with 5% sheep blood and hemoglobin, adhere to CLSI standards, ensuring reliability and reproducibility. Trust TM Media for all your microbiological needs, where excellence meets your laboratory requirements. Visit our website for more information.
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