For cold enrichment and recovery of Yersinia enterocolitica and Yersinia pseudotuberculosis from foods. Dissolve 26.30 gms in 900ml of distilled water. Gently heat to boiling with gentle swirling and dissolve the medium completely. Sterilize by autoclaving at 15 psi (121ºC) for 15 minutes. Cool to 20-25°C and aseptically add 100 ml of 4% filter sterilized sorbose solution. Mix well and dispense as desired. - Appearance: Reddish pink coloured clear solution- : PH (at 25°C): 7.9 ± 0.2
YEAST EXTRACT ROSE BENGAL BROTH BASE is used for cold enrichment and recovery of Yersinia enterocolitica and Yersinia pseudotuberculosis from foods. Yersinia enterocolitica causes several diseases, especially in younger persons, and several uncommon post-infection syndromes. Yersinia pseudotuberculosis causes a zoonotic disease with its natural reservoir being rodents, wild animals and game birds. Yersinia species are psychrotropic and therefore grow at 4°C. Primary enrichment for Yersinia enterocolitica and Yersinia pseudotuberculosis is carried out in Yeast Extract Rose Bengal Broth Base for 3 days at 10°C or 9 days at 4°C. Yeast extract provides essential nutrients. Bile salts inhibit gram-positive organisms. Various salts help in cold enrichment of Yersinia species.