SKU: sku-2334
Intended Use: 
TCBS Agar is used for selective isolation of Vibrio cholerae & other enteropathogenic Vibrios causing food poisoning.
Product Code: 
TM 436
Principles: 
TCBS AGAR (VIBRIO SELECTIVE AGAR) is highly selective for the isolation of Vibrio cholerae and V. parahaemolyticus from stool specimens, and specified in standard methods for food testing. This highly selective medium meets the nutritional requirements of Vibrio species and allows competing with intestinal flora. Vibrio species is able to grow in media containing increased salt concentrations. Vibrio species are natural inhabitants of water. Proteose peptone and Yeast extract are the sole sources of carbon, nitrogen, vitamin B - complex, minerals and amino acids in the medium. Sodium thiosulphate serves as a sulphur source and, in combination with Ferric citrate, detects hydrogen sulphide production. Sucrose is included as a fermentable carbohydrate for the metabolism of Vibrio. Inhibition of gram- positive bacteria is achived by the incorporation of Ox bile, which is a synthetic occurring substance and suppresses primarily Enterococci. Ox bile also helps to inhibit gram - positive bacteria.

Product Category:

Product Description: 
  1. TCBS  AGAR  (VIBRIO  SELECTIVE  AGAR)  is highly selective for the isolation of Vibrio cholerae and V.parahaemolyticus from stool specimens and specified in standard methods for food testing.    
  2. This highly selective tcbs medium meets the nutritional requirements ofVibriospeciesand allows competing with intestinal flora. Vibrio species is able to grow in media containing increased salt concentrations. Vibrio species are natural inhabitants of the water.      
  3. Proteose peptone and Yeast extract is the sole source of carbon, nitrogen, vitamin B -complex, minerals and amino acids in the medium.  Sodium thiosulphate serves as a  sulphur source and,  in combination with   Ferric citrate,   detects hydrogen sulphide production.   Sucrose is included as a   fermentable carbohydrate for the metabolism of Vibrio.  Inhibition of gram-positive bacteria is achieved by the incorporation of Ox bile, which is a synthetic occurring substance and suppresses primarily Enterococci. Ox bilealso helps to inhibit gram -positive bacteria.       
  4. The alkaline pH of the medium enhances the recovery of V. cholera because this organism is sensitive to acid environments.  Thymol blue and  Bromothymol blue are included as indicators of pH changes. Sodium citrate and  Sodium thiosulphate are the selective agents,  providing an alkaline pH to inhibit gram-positive organisms and suppress coliforms.  Agar is the solidifying agent.  TCBS  Agar deeps  (pour tubes)  are provided in a 20  ml fill so that the medium may be liquefied and poured into Petri plates. This provides a convenient source of medium with a longer shelf -life than pre-poured plated media.