Spirit Blue Agar is used for detection and enumeration of lipolytic microorganisms.
SPIRIT BLUE AGAR is used for detection and enumeration of lipolytic microorganism. Spirit Blue Agar is prepared according to the formulation of Starr. It is a basal medium to which lipoidal substrate is added for the detection, enumeration and study of lipolytic microorganisms. Formulations in practice before Starr which included dyes as indicators of lipolysis were sometimes inhibitory to the microorganisms. Starr showed spirit blue to be inert and an ideal indicator of lipolysis, visualized as clear halos around colonies.