Every sample tested in a microbiology lab carries a mystery: invisible microorganisms waiting to be identified. The story begins the moment a plate of soybean casein digest agar is poured. In a matter of hours, those invisible microbes become colonies that provide information about the safety and cleanliness of a product. This is the quiet power of Microbiology tests turning what can’t be seen into what can be measured, understood, and controlled.
Soybean Casein Digest Agar, also known as Tryptic Soy Agar (TSA), is a nutrient-rich medium that supports the growth of a wide variety of microorganisms. The medium contains enzymatic digests of casein and soybean as sources of amino acids, a source of nitrogen and various other growth factors for the microorganisms.
It is excellent for testing, counting and holding culture for both aerobic and facultative anaerobic bacteria. Its flexibility and reliability have made it a staple in every microbiology assay.
Each microbiology test relies on the medium’s ability to support growth. Soybean Casein Digest Agar attains this by a balanced nutrient composition:
Such a combination creates an environment rich enough to enable lab professionals to confidently identify the smallest indications of microbial contamination.
The power of soybean casein digest agar lies in its balanced formulation. It is specifically designed for the growth of a wide range of organisms:
| Ingredient | Amount (g/L) |
| Pancreatic digest of casein | 15.0 |
| Papaic digest of soybean | 5.0 |
| Sodium chloride | 5.0 |
| Agar | 15.0 |
The nutrient-rich composition results in luxuriant growth of bacteria, fungi, and yeast, which is essential for a successful microbiological test in the pharmaceutical and industrial laboratories.
Preparing the medium is simple yet requires accuracy because consistency determines the reliability of your microbiology test results:
After preparation the medium is pale yellow and solidifies evenly and can be used for the cultivation of various microorganisms.
Each batch of soybean casein digest agar undergoes strict quality control to assure uniformity and reliability in the results of the microbiology tests. During testing, laboratories will find dependable growth of many different microorganisms, such as:
| Microorganism | ATCC | Inoculum (CFU/ml) | Growth | Recovery | Incubation Temperature | Incubation Period |
| Staphylococcus aureus | 6538 | 50-100 | Luxuriant | ≥ 70% | 30-35°C | 18-24 hours |
| Staphylococcus aureus | 25923 | 50-100 | Luxuriant | ≥ 70% | 30-35°C | 18-24 hours |
| Escherichia coli | 8739 | 50-100 | Luxuriant | ≥ 70% | 30-35°C | 18-24 hours |
| Escherichia coli | 25923 | 50-100 | Luxuriant | ≥ 70% | 30-35°C | 18-24 hours |
| Pseudomonas aeruginosa | 9027 | 50-100 | Luxuriant | ≥ 70% | 30-35°C | 18-24 hours |
| Pseudomonas aeruginosa | 27853 | 50-100 | Luxuriant | ≥ 70% | 30-35°C | 18-24 hours |
| Bacillus subtilis | 6633 | 50-100 | Luxuriant | ≥ 70% | 30-35°C | 18-24 hours |
| Salmonella typhimurium | 14028 | 50-100 | Luxuriant | ≥ 70% | 30-35°C | 18-24 hours |
| Klebsiella pneumoniae | 13883 | 50-100 | Luxuriant | ≥ 70% | 30-35°C | 18-24 hours |
| Enterococcus faecalis | 29212 | 50-100 | Luxuriant | ≥ 70% | 30-35°C | 18-24 hours |
| Streptococcus pneumoniae | 6305 | 50-100 | Luxuriant | ≥ 70% | 30-35°C | 18-24 hours |
| Micrococcus luteus | 9341 | 50-100 | Luxuriant | ≥ 70% | 30-35°C | 18-24 hours |
| Clostridium sporogenes | 19404 | 50-100 | Luxuriant | ≥ 70% | 30-35°C | 18-24 hours |
| Candida albicans | 10231 | 50-100 | Luxuriant | ≥ 70% | 30-35°C | <=5 days |
| Aspergillus brasiliensis | 16404 | 10-100 | Good-Luxuriant | 50-70% | 30-35°C | <=5 days |
In the crowded world of microbial testing, TM Media’s quality assured soybean casein digest agar (TMH 103) stands out for all the right reasons:
To those labs doing the same daily microbiology work, this is not just a consumable medium, it is a staple companion in that journey towards accuracy.
In the precise world of microbiology testing, accuracy begins with the medium you select. Soybean Casein Digest Agar (TMH 103) is the result of years of experience and continuous improvement delivering quality, which enables scientist and quality controllers best support in each and every microbiology test they conduct.
From the verification of sterility to the conduct of any microbial testing procedure, TM Media delivers precision and reliability in every result.
Because, in microbiology, every colony tells a story and with soybean casein digest agar, it is always a story of confidence and control.
A. Yes. Its formulation is compatible with automation for microbial counts and detection and enables seamless, consistent processing of high-throughput microbiology samples.
A. Generally, prepared plates can be stored for up to two weeks under refrigeration. But before being used for microbiology testing, they always should be inspected for drying out, contamination or colour changes.
A. Deterioration may be indicated by discolouration, caking, or wetting. Such a medium should never be employed for microbiological testing, because it may lead to false or unreliable results.
A. It allows luxuriant growth of both gram-positive and gram-negative bacteria.
A. Yes, it grows Candida albicans and Aspergillus brasiliensis and it can also be used for combined bacterial and fungal microbiology testing in a single setup.
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