Understanding Murashige and Skoog Media for Plant-Tissue culture

Published: 23rd Apr 2024, 13:39

In plant tissue culture, Murashige & Skoog Medium, commonly known as MS Medium, stands as an iconic formulation that has revolutionized the field since its inception in 1962. Crafted by the pioneering efforts of Murashige and Skoog, this medium has become synonymous with excellence in nurturing plant cells and facilitating a variety of tissue culture techniques.

Components of MS Media

At the heart of the MS Medium’s success lies its precise formulation, featuring a balanced blend of essential elements crucial for supporting plant growth and proliferation in both monocotyledons and dicotyledons. Let us take a closer look at the key ingredients and their concentrations in the MS Medium:

IngredientConcentration (Mg/L)
Potassium hydrogen phosphate170.000
Potassium nitrate1900.000
Magnesium sulphate180.680
Ammonium nitrate1650.000
Cobalt chloride.6H2O0.025
Copper sulfate.5H2O0.025
Boric acid6.200
Ferrous sulfate.7H2O27.80
Potassium iodide0.830
EDTA disodium salt.2H2O37.30
Manganese sulfate.H2O16.900
Sodium molybdate0.250
Zinc sulfate.7H2O8.600
Nicotinic acid0.500
Pyridoxine hydrochloride0.500
Thiamine hydrochloride0.100

The accurate balance of these components creates an environment that nurtures plant cells, allowing them to thrive and proliferate.

Applications of MS Medium in Plant Research:

MS Medium finds widespread applications in various plant research endeavours. Here are some key areas where MS Medium plays a crucial role:

  1. Micropropagation: MS Medium provides an ideal environment for the rapid multiplication of plants through micropropagation, allowing researchers to produce many genetically identical plants from a single parent plant.
  2. Organ Culture: Organ culture involves the growth of entire plant organs in vitro. MS Medium’s balanced formulation supports the development of organs such as roots, shoots, and leaves under controlled conditions.
  3. Callus Culture: MS Medium is instrumental in callus culture, where undifferentiated plant cells are cultured to form a mass of cells. This technique is often used in genetic engineering and the production of secondary metabolites.
  4. Suspension Culture: Suspension culture involves growing plant cells freely suspended in the medium. MS Medium provides the necessary nutrients for the maintenance of cell suspension cultures, enabling the study of plant cell physiology and biochemistry.

The Murashige & Skoog Medium, formulated by TM Media, has a rich history of nurturing plant tissue cultures and providing researchers with an ideal environment for their experiments. Its precise formulation and ease of use make it a dependable choice for those exploring the wonders of plant growth in various research fields, including plant and agriculture research. Whether you are engaged in micropropagation, organ culture, callus culture, or suspension culture, MS Medium is here to help you cultivate success in your plant tissue culture endeavours. TM Media is the leading name for any of your plant-tissue culture needs. For more information, please visit our website.


The Murashige & Skoog Medium (MS medium) has been a revolutionary force in plant tissue culture since 1962. Crafted by Murashige and Skoog, its precise formulation supports plant growth with a balanced blend of essential elements. The step-by-step preparation guide ensures its effectiveness, making it a go-to for various applications like micropropagation, organ culture, callus culture, and suspension culture in plant research. MS Medium, with its storied history, remains a vital tool in nurturing plant cells and advancing scientific endeavours.Top of Form

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