T.A.T. BROTH BASE

Product Code TM 435

  • Description

    T.A.T. Broth is prepared according to the formula recommended by United States Food and Drug Administration for enrichment and further isolation and cultivation of gram-negative bacteria in cosmetics, tropical drugs and in the sterility testing of viscous or gelatinous substances. It is especially adapted for the testing of cosmetics. Cosmetics and pharmaceutical products are subject to contamination during manufacturing and subsequent use by consumers. Preservatives are used in aqueous products to make them self-sterilizing for vegetative bacteria, yeasts and moulds, and bacteriostatic or bactericidal for spores. Prepare decimal dilutions of the sample to be tested from 10-1 to 10-6. Inoculate 1 gram (1 ml) sample and 1 ml of each dilution into 40 ml of T.A.T. Broth. After incubation, subculture the growth on MacConkey Agar and TSI Agar.

  • Principle

    for sterility testing of highly viscous substances such as ointments, salves and other cosmetic products

  • Microorganism

    • Bacillus subtilis
    • Candida albicans
    • Pseudomonas aeruginosa
    • Salmonella typhi
    • Staphylococcus aureus
  • Industry

    • NA
  • Regulation

    • NA
  • Pack Size

    • 500 gm
  • Downloads

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