SYNTHETIC BROTH, AOAC (WRIGHT AND MUNDY BROTH)

The phenol coefficient test has been employed for determining the germicidal efficiency of disinfectants for over 30 years since Rideal and Walker developed their original method in 1903. In addition to being a satisfactory index of the germicidal value of phenol like disinfectants, the phenol coefficient is used as basis for determining the dilutions, which may safely be employed in practice. The phenol coefficient of each disinfectant was first determined by the Food and Drug Administration method, 1931. Since then there is no standard method for testing disinfectants under practical conditions. Synthetic Broth is prepared, based on the formulation described by Wright and Mundy and is recommended by AOAC for determining phenol coefficients of disinfectants.

for growing inoculum, making subcultures and preparing various dilutions while testing disinfectants