SOC BROTH

SOC Broth Base is a medium which is prepared by adding 20% glucose solution to SOB Medium (Hanahans Broth). This medium is a nutritionally rich growth medium used for growing bacterial cells, for preparing chemically competent cells and in the recovery step of competent cell transformations. E. coli is first grown in SOB Medium (Hanahans Broth) to get the desired cell density. The cells are then harvested and subjected to chemical treatment or electoporation to develop competent cells. These competent cells are then transformed using suitable method. The transformants are then grown in SOC Medium. The use of SOC Broth maximizes the transformation efficiency of competent cells.

a medium for molecular biology