SELENITE BROTH (SELENITE F BROTH) (DOUBLE PACK) (PART – I)

Klett first demonstrated the selective inhibitory effects of selenite and Guth used it to isolate Salmonella typhi. Leifson fully investigated selenite and formulated the media. Enrichment media are routinely employed for detection of pathogens in faecal specimens as the pathogens are present in a very small number in the intestinal flora. Selenite Broth is useful for detecting Salmonella in the nonacute stages of illness when organisms occur in the faeces in low numbers and for epidemiological studies to enhance the detection of low number of organisms from asymptomatic or convalescent patients. It is recommended by BIS committee under specification IS: 5887 (Part III) 1999, reaffirmed 2005.

for isolation and enrichment of Salmonella from faeces, urine or other pathological material.