MacCONKEY BROTH MEDIUM 7.

MacConkey Broth is a modification of MacConkey Medium. Childs and Allen demonstrated the inhibitory effect of neutral red and therefore substituted it by the less inhibitory bromocresol purple dye. BCP is more sensitive in recording pH variation in the medium. Indian pharmacopoeia has recommended this medium for the presumptive identification of coliforms from water and other materials of sanitary importance. Transfer homogenate in Casein Soyabean Digest Broth containing 1 gm or 1 ml of the preparation to be examined to 100 ml MacConkey Broth Incubation is carried at 43?-45?C for 24-48 hours. For further isolation subculture on MacConkey Agar. Growth of red generally non-mucoid colonies, sometimes surrounded by a reddish precipitation zone, indicates pressure of coliforms.

for the selective identi?cation of E.coli from pharmaceutical products in accordance with Indian Pharmacopoeia 2014