M-BISMUTH SULPHITE BROTH

Salmonella is a gram-negative, non-sporulation, facultative anaerobic, non-motile rod in the family Enterobacteriaceae. They are widely distributed in animals causing diseases mainly in stomach and the intestines. It is difficult to differentiate these organisms biochemically from Escherichia coli. Clark et al formulated M-Bismuth Sulphite Broth and recommend for detection of Salmonella Typhi by the membrane filtration technique from water and various clinical specimens. Preliminary enrichment on a non-selective medium is not necessary. M-Bismuth Sulphite Broth share same composition with Bismuth Sulphite Agar except Agar. All the constituents are in double concentration in the broth medium.

for the detection of Salmonellae by the membrane ?lter technique