Loeffler Medium was originally devised by Loeffler and was further modified by Perry and Petran and Buck. Loeffler medium enhances primary and secondary isolation and cultivation of fastidious pathogenic microorganisms especially from nose and throat. It also restores virulence and other identifying properties (microscopic and colonial) after they have been lost due to prolonged incubation or repeated sub culturing. The high serum content helps in determining proteolytic activity of organisms. It is also used for demonstration of pigmentation and ascospores.

for cultivation of Corynebacterium diffhtheriae from clinical samples and confirmation of chromogenesis, proteolysis and the production of ascospores