L.J. MEDIUM MODIFIED

Solid media used for isolation and cultivation of Mycobacteria are either egg-based or agar-based. Egg-based media contain whole eggs or egg yolk, potato flour, salts and glycerol and are solidified by inspissation. Of the egg-based media, Lowenstein Jensen Medium is most commonly used.
L.J. Medium was originally formulated by Lowenstein, containing congo red and malachite green dyes. Jensen modified Lowensteins medium by altering the citrate and phosphate contents, eliminating the congo red dye and by increasing the malachite green concentration. This medium supports the growth of a wide variety of Mycobacteria and can also be used for niacin testing.

used for the isolation of Mycobacterium species from mixed flora