EMB AGAR, LEVINE PLATE

Product Code TMP 025

  • Description

    Levine EMB Agar was developed by Levine and is used for the differentiation of Escherichia coli and Enterobacter aerogenes and also for the rapid identification of Candida albicans. This medium is recommended for the detection, enumeration and differentiation of members of the coliform group by American Public Health Association. Weld proposed the use of Levine EMB Agar, with added Chlortetracycline hydrochloride, for the rapid identification of in clinical specimens. A positive identification of Candida albicans can be made after 24 – 48 hours incubation at 35 – 37?C in 10% carbon dioxide atmosphere, from specimens such as faeces, oral and vaginal secretions and nail or skin scraping etc. However, the typical appearance is variable.
    Eosin Y and methylene blue make the medium slightly selective and inhibit certain gram-positive bacteria. These dyes serve as differential indicators in response to the fermentation of carbohydrates. This helps to differentiate between lactose-fermenters and non-fermenters in EMB Agar, Levine. The ratio of eosin-methylene blue is adjusted to approximately 6:1. Coliforms produce purplish black colonies due to uptake of methylene blue-eosin dye complex, when the pH drops. The dye complex is absorbed into the colony. Non-fermenters probably raise the pH of surrounding medium by oxidative de-amination of protein, which solubilizes the methylene blue-eosin complex resulting in formation of colourless colonies. Some strains of Salmonella and Shigella species do not grow in the presence of eosin and methylene blue.

  • Principle

    For isolation, enumeration and di?erentiation of Enterobacteriaceae.

  • Microorganism

    • Candida albicans
    • Enterobacter aerogenes
    • Escherichia coli
    • Pseudomonas aeruginosa
    • Saccharomyces cerevisiae
    • Salmonella serotype Typhimurium
    • Staphylococcus aureus
  • Industry

    • Clinical Diagnostics
  • Regulation

    • NA
  • Pack Size

    • 50 Plts
  • Downloads

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