COLUMBIA BROTH BASE

Product Code TM 073

  • Description

    Morello and Ellner in 1969 devised a liquid medium for the recovery of microorganisms from blood cultures. This medium was devised from Columbia Blood Agar Base previously formulated by Ellner et al. While studying they found that Columbia Broth was superior to a commonly used general-purpose broth for faster growth of Staphylococcus aureus, Escherichia coli, viridans Streptococci and Enterococcus groups. In the formulation the increased concentration of cystine is provided for improved recovery of both aerobic and anaerobic microorganisms from blood specimens. Columbia Broth Base supplemented with SPS (Sodium Polyanethol Sulphonate), a polyanionic anticoagulant inhibits complement and lysozyme activity, interferes with phagocytosis and inactivates aminoglycosides. The presence of CO2 is stimulatory for many organisms. It is an excellent blood culture medium. It differs from the agar base in that the cornstarch is omitted to reduce opalescence and salts have been included.
    Tube media should be inoculated with 1 to 2 drops of the liquid specimen using a sterile pipette. Swab specimens may be inserted into the broth after inoculation of the plated media. Liquid media should be reduced by placing the tubes with caps loosened under anaerobic conditions for 18-24 hours prior to inoculation for anaerobic incubation. Alternatively, it can be reduced immediately before use by boiling with caps loosened and cooling to room temperature with tightened caps, before inoculation. Growth in tubes is indicated by presence of turbidity compared to an uninoculated control. If growth appears, cultures should be subcultured onto appropriate media. Addition of SPS is inhibitory to Neisseria species, and thus 1.2% gelatin addition may counteract the inhibitory effect.

  • Principle

    for cultivation of fastidious microorganisms from clinical sources

  • Microorganism

    • Clostridium perfringens
    • Neisseria meningitidis
    • Staphylococcus aureus subsp. aureus
    • Streptococcus mitis
    • Streptococcus pyogenes
  • Industry

    • NA
  • Regulation

    • NA
  • Pack Size

    • 500 gm
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