CHROMOGENIC UNIVERSAL DIFFERENTIAL MEDIUM

Chromogenic Universal Differential Medium is a modification of the medium formulated on basis of the work carried out by Pezzlo, Wilkie et al, Friedman et al, Murray et al, Soriano and Ponte and Merlino et al. Chromogenic Universal Differential Medium is recommended for the presumptive identification of microorganisms from clinical and no clinical specimens where the medium has broader application as a general nutrient agar for isolation of various microorganisms. This medium helps in the identification of some gram-positive bacteria and gram-negative bacteria on the basis of different colony colors exhibited by them. These colors are formed due to the reactions of genus or species specific enzymes with the two chromogenic substrates incorporated in the medium. Enterococcus species, Escherichia coli and coliforms produce enzymes which specifically cleave these chromogenic substrates to give characteristically distinctive colony colors.

recommended for presumptive identi?cation of microorganisms from clinical and non-clinical specimens