Product Code TM 2035

  • Description

    Cetrimide Agar Base w / 1.3% Agar is recommended as a selective medium for isolation of Pseudomonas aeruginosa. It is similar in composition as cited in various pharmacopoeias except that the concentration of agar in this medium is 1.3%. The original formula was described by King et al. It can also be used for determining the ability of an organism to produce fluorescein and pyocyanain.
    King et al developed Medium A for the enhancement of pyocyanin production by Pseudomonas. Cetrimide agar developed by Lowburry is a modification of Tech Agar (Medium A) with addition of 0.1% cetrimide for selective isolation of P. aeruginosa. Later, due to the availability of the highly purified cetrimide, its concentration in the medium was decreased. The incubation was carried out at 37?C for a period of 18-24 hours. P. aeruginosa can be identified due to their characteristic production of pyocyanin, a blue, water soluble, nonfluorescent phenazine pigment coupled with their colonial morphology and the characteristic grape like odour of aminocetophenone.
    For the isolation of P. aeruginosa, plates of cetrimide agar should be inoculated from non-seelctive medium such as Brain Heart infusion Broth or Soyabean Casein Digest Medium.If the count is high, the test sample can be directly inoculated onto Cetrimide Agar. P. aeruginosa colonies may appear blue, blue-green or nonpigmented. Colonies exhibiting fluorescence at 250 nm and a blue green pigmentation are considered as presumptive positive. P. aeruginosa may lose its fluorescence under UV if the cultures are left at room temperature for short time. Fluorescence reappears after the plates are re-incubated. Goto and Enomoto recommended that addition of nalidixic acid aids in inhibiting the growth of accompanying flora.

  • Principle

    for the selective isolation of Pseudomonas aeruginosa from various materials

  • Microorganism

    • Pseudomonas aeruginosa
  • Industry

    • Pharmaceutical
    • Water
  • Pack Size

    • 100 gm
    • 500 gm
  • Downloads

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