ASHBY?S GLUCOSE AGAR

Azotobacter is a genus of free-living diazotrophic bacteria which have the highest metabolic rate compared to any other microorganism. Azotobacters are chemoorganotrophic, using sugars, alcohols and salts of organic acids for growth. Ashby’s Agar Media are formulated as described by Subba Rao. It is used for isolation of Azotobacter, a non-symbiotic nitrogen fixing bacteria which uses glucose as a carbon source and atmospheric nitrogen as nitrogen source. Besides the ability to fix atmospheric nitrogen, Azotobacter also synthesize biologically active substances which attributes to improving seed germination, plant growth etc.

for cultivation of Azotobacter species by using glucose as carbon source