ANTIBIOTIC ASSAY MEDIUM NO. 35

This medium is formulated in accordance to CFR. This medium is employed widely as base agar for agar diffusion assay of Bleomycin using Mycobacterium smegmatis.
Addition of glycerol is important for provision of carbon to the test organism. To perform the antibiotic assay, the Base Agar should be prepared on the same day as the test. For the cylinder method, a base layer of 21 ml is required. Once the base medium has solidified, seed layer inoculated with the standardized culture can be overlaid. Even distribution of the layer is important

for microbiological assay of Bleomycin using Mycobacterium smegmatis