ACETAMIDE BROTH (DOUBLE PACK)

A wide variety of pathogenic microorganisms can be transmitted to humans through use of natural fresh and marine recreational waters contaminated by waste water. Pseudomonas aeruginosa is one of the organisms that are capable of growth in water at very low concentrations of nutrients. While the primary indicators of water quality are Escherichia coli and Enterococci, the enumeration of Pseudomonas aeruginosa in recreational waters may be useful in cases of discharge of pulp and paper wastes and effluents from textile finishing plants into receiving waters. One of the unique properties of P. aeruginosa is its ability to produce ammonia from acetamide.
Acetamide Broth, formulated as per DRAFT prEN 12780:1999 is recommended for the confirmation of non-fermentative gram-negative Pseudomonas aeruginosa. Organisms growing in this medium metabolize acetamide by process of deamination (acrylamidase activity). This ability is shown by Ps. aeruginosa, Ps. acidovorans Group III (Achromobacter xylosoxidans) and Alcaligens odorans.
The test water samples are filtered through sterile cellulose ester membrane filters. These filters are aseptically placed on Pseudomonas Agar Base containing Cetrinix Supplement. These plates with filters are incubated at 35- 37?C for 24-48 hours. Pyocyanin-producing colonies are counted as confirmed Ps.aeruginosa . Non-pyocyanin- producing fluorescent colonies are counted as presumptive Ps.aeruginosa . These presumptive Ps.aeruginosa colonies are confirmed by using Acetamide Broth. Production of ammonia from acetamide can be detected by the addition of Nesslers reagent.

for con?rmation of non-fermentative Gram-negative bacteria, particularly Pseudomonas aeruginosa