For cultivation of Agrobacterium species and other soil microorganisms. YEM broth is widely used for the cultivation of Agrobacterium species and other soil microorganisms. It is also widely used for the cultivation of the symbiotic nitrogen fixing microorganisms like Rhizobium species to make it suitable for the production of legume inoculants.This Medium contains mannitol as a carbon source and yeast extract as a source of both nitrogen and growth factors for Agrobacteria. It also poises oxidation - reduction potential of medium in the range favorable for Rhizobia and serves as hydrogen donor in respiratory process. Mannitol is the fermentable sugar alcohol source. Magnesium provides cations essential for the growth of Agrobacteria.