For isolation and differentiation of Gardenerella vaginalis from clinical samples. Vaginalis Agar Base used to isolate Gardenerella vaginalis, responsible for bacterial vaginosis (BV). BV is the most common cause of vaginitis and the most common infection encountered in the outpatient gynaecological setting. Generally, Columbia Agar Base media with added sheep blood is used for culturing human pathogenic microflora. Since, G. vaginalis exhibits no hemolysis on sheep blood; it was replaced by human blood in Vaginalis Agar Base, as G. vaginalis exhibits beta-hemolysis on human blood.
The medium consists of peptic digest of animal tissue, casein enzymic hydrolysate, beef extract and yeast extract as the sources for nitrogen, carbon, vitamins and trace elements required for organism growth. Corn starch is added as an energy source. Sodium chloride maintains the osmotic balance. Agar acts as a solidifying agent. Blood supplies additional nutrients and also aids in identification. Typical colonies of G. vaginalis appear small and white coloured. This medium is recommended for determin ation of haemolytic reaction of G. vaginalis and not for other microorganisms. If the specimen is suspected to contain streptococci or other haemolytic microorganisms, then a Soyabean Casein Digest Agar (with 5% v/v sheep blood) plate should be inoculated parallel to this medium to ensure the haemolytic reaction.