TRYPTOSE CYCLOSERINE AZIDE AGAR BASE

Tryptose Cycloserine Azide Agar Base was originally formulated by Hauschild and Hilsheimer. This medium was later modified by decreasing the concentration of D-cycloserine, sulphite and iron and by the addition of sodium azide . This medium utilizes the selective inhibitory properties of D-cycloserine and an indicator system involving sulphite and iron. Growth of non-mesophillic organisms are suppressed while C. perfringens and related species will reduces the sulphite and form black colonies due to the production of ferrous sulphide.

for enumeration of sulphite reducing anaerobes essentially Clostridia