For isolation of Trichomonas vaginalis
TRICHOMONAS BROTH BASE NO.2 is used for isolation of Trichomonas vaginalis. Stenton reported that incorporation of liver digest in the medium plays an important role in detection of Trichomonas vaginalis. The medium is equally suitable for the examination of urethral and vaginal swabs and urine specimens. Casein enzymic hydrolysate and papaic digest of soyabean meal and liver digest provide the nitrogenous substances. Glucose acts as the energy source. The selective agent chloramphenicol is inhibitory to gram-positive and gram-negative bacteria but not for Trichomonas species. Sodium chloride maintains the osmotic equilibrium of the medium. Dipotassium phosphate buffers the medium. Calcium pantothenate acts as a growth factor. The small amount of agar helps to create anaerobiosis. Addition of Horse serum makes the medium highly nutritious got T. vaginalis.