SKU: sku-2335
Intended Use: 
for selective isolation of Vibrio cholerae & other enteropathogenic Vibrios causing food poisoning. TCBS AGAR (VIBRIO SELECTIVE AGAR) (VEG.) is highly selective for the isolation of Vibrio choleraeand     V. parahaemolyticus from stool specimens, and specified in standard methods for food testing. This highly selective medium meets the nutritional requirements of Vibrio spp., and allows competing with intestinal flora. Vibrio spp. is able to grow in media containing increased salt concentrations. Vibrio spp. are  natural  inhabitants  of  water.  Veg.  PP  peptone  and  Yeast  extract  are  the  sole  sources  of  carbon, nitrogen, vitamin B - complex, minerals and amino acids in the medium. Sodium thiosulphate serves as asulphur source and, in combination with Ferric citrate, detects hydrogen sulphide production. Sucrose is included  as  a  fermentable  carbohydrate  for  the  metabolism  of  Vibrios.  Inhibition  of  gram-  positive bacteria is achived by the incorporation of Synthetic detergent, which is a synthetic occurring substance and suppresses primarily Enterococci. Synthetic detergent, also helps to inhibit gram - positive bacteria. The alkaline pH of the medium enhances the recovery of V. cholera because this organism is sensitive to acid  environments.  Thymol  blue  and  Bromothymol  blue  are  included  as  indicators  of  pH  changes. Sodium  citrate  and  Sodium  thiosulphate  are  the  selective  agents,  providing  an  alkaline  pH  to  inhibit gram- positive organism and suppress coliforms. Agar is the solidifying agent. TCBS Agar deeps (pour tubes) are provided in a 20 ml fill so that the medium may be liquefied and poured into a petri plates. This provides a convenient source of medium with a longer shelf - life than pre- poured plated media.
Product Code: 
TMV 436