SKU: sku-2336
Intended Use: 
TCBS AGAR is used for selective isolation of Vibrio cholerae and other enteropathogenic Vibrios.
Product Code: 
TM 875

Product Category:

Product Description: 

TCBS AGAR (VIBRIO SELECTIVE AGAR) ishighly selective for the isolation of Vibrio choleraeandV.parahaemolyticusfrom  stool  specimens,  and  specified  in  standard  methods  for  food  testing.  This  highly selective  medium  meets the  nutritional requirements of Vibriosp.,  and  allows competing  with  intestinal flora. Vibriospp. is able to grow in media containing increased salt concentrations. Vibrio spp. are natural inhabitants  of  water.  Special  peptone  and Yeast  extractarethe  source of vitamins,  carbon,  nitrogen  and amino  acids  in  the  medium. Sodium  thiosulphate  serves  as  a  sulphur  source  and,  in  combination  with Ferric citrate,  detects  hydrogen  sulphide  production. Sucrose  is  included  as  a  fermentable  carbohydrate for  the  metabolism  of  Vibrios.  Inhibition  of  gram-positive  bacteria  is  achived by  the  incorporation  of Oxgall, which is a naturally occurring substance containing a mixture of bile salts, and Sodium cholate, a pure  bile  salt.  Oxgalland  cholate  suppress  primarily  enterococci.  The  alkaline  pH  of  the  medium enhances the recovery of V. cholera becausethis organism is sensitive to acid environments. Thymol blue and   Bromothymol   blue   are   included   as   indicators   of   pH   changes.   Sodium   citrate   and   Sodium thiosulpahte  are  the  selective  agents,  providing  an  alkaline  pH  to  inhibit  gram-positive  organims  and suppress coliforms. Agar is the solidifying agent. TCBS agar deeps (pour tubes) are provided in a 20 ml fill so that the medium may be liquefied and poured into a petri plates. This provides a convenient source of medium with a longer shelf -life than pre-poured plated media.