SKU: sku-2338
Intended Use: 
for selective isolation of Vibrio cholerae and other enteropathogenic Vibrio's. It is recommended by BIS committee under specification IS: 5887 (Part V) 1976, reaffirmed 2005. Thiosulfate citrate-bile salts sucrose agar ishighly  selective  for  the  isolation of Vibrio  choleraeandV.parahaemolyticusfrom  stool specimens,  and  specified  in standard  methods  for  food  testing  as  per recommended  by BIS.  This  highlyselective medium meets the nutritional requirements of Vibriospp., and allows competing with intestinal flora. Vibriospp. isable to grow in media containing increased salt concentrations. Vibrio spp. are natural inhabitants of water. Proteose peptoneand Yeast extractarethe sole sourcesof carbon, nitrogen, vitamin B -complex,minerals and  amino  acids  in  the  medium.  Sodium thiosulphate serves  as  a  sulphur  source and,  in  combination  with  Ferric  citrate,  detects  hydrogen  sulphide  production.  Sucrose  is  included  as  a fermentable carbohydrate  for  the  metabolism  of  Vibrios.  Inhibition  of  gram-positive  bacteria  is  achived by  the incorporation  of Ox  gall,  which  is  a  synthetic  occurring  substance  and  suppresses  primarily Enterococci.  Ox  gall  also  helps  to  inhibit  gram -positive  bacteria.  The  alkaline  pH  of  the  medium enhances the recovery of V. cholera becausethis organism is sensitive to acid environments. Thymol blue and   Bromothymol   blue   are   included   as   indicators   of   pH   changes.   Sodium   citrate   and   Sodium thiosulphate  are  the  selective  agents,  providing  an  alkaline  pH  to  inhibit  gram-positive  organism  and suppress coliforms. Agar is the solidifying agent. Thiosulfate citrate-bile salts sucrose agar deeps (pour tubes) are provided in a 20 ml fill so that the medium may be liquefied and poured into a petri plates. This provides a convenient source of medium with a longer shelf -life than pre-poured plated media.
Product Code: 
TM 1299