For selective isolation and enumeration of Clostridium perfringens from foodstuffs. Dissolve 41.28gms in 1000ml of distilled water. Gently heat to boiling with gentle swirling and dissolve the medium completely. Sterilize by autoclaving at 15 psi (121 ºC) for 15 minutes. Appearance: Medium amber coloured slightly opalescent -: pH (at 250C): 7.0 ± 0.2
SPS AGAR, MODIFIED is used for selective isolation and enumeration of Clostridium perfringens from foodstuffs. This medium was first modified by Angelotti, which is based on the theory of Wilson and Blair Medium. On incorporating Polymyxin B sulphate and Sulphadiazine to the most recent Mossel formula for the recovery of Clostridium perfringens. The medium contains Casein enzymatic hydrolysate and Yeast extract which provides nitrogen and essential nutrients, vitamin B group to the medium. Agar is the solidifying agent. Ferric citrate and Sodium sulfite are H2S indicators. Polymyxin B and sulphadiazine inhibit a wide variety of gram-positive and gram-negative bacteria. Incubate the plates anaerobically at 35 - 37°C for 18 - 48 hours.