For detection of microorganisms in blood. TRYPTONE SOYA BROTH or SOYA CASEIN DIGEST MEDIUM (SCDM) is used for the sterility testing, cultivation of fastidious and non- fastidious microorganisms. The medium is used for a multitude of purposes including maintenance of stock cultures, plate counting and isolation of microorganisms from a variety of specimen types and as a base for media containing blood. Pancreatic digest of casein and Soyatone supply the nitrogen and carbon source to the medium. Dextrose is a source of fermentable carbohydrates for energy production. Sodium chloride maintains the osmotic balance and Dipotassium phosphate provides buffering capacity in the medium. Clostridia sp. and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. SCDM is also used for testing bacterial contaminants in cosmetics, also in the food industry, and used for detecting viable bacteria in live vaccines. SCDM is also used for inoculum preparation in disk diffusion sensitivity tests. The rich nutritional base of SCDM, supplemented with SPS and CO2, is an excellent broth for blood cultures in clinical applications. The growth of the microorganisms tested (about 103 – 105 CFU/ml) shown to have positive and luxuriant colonies, after incubation period of 24 hours extended to 3 - 5 days at 30º - 35ºC.