SEMISOLID RAPPAPORT VASSILIADIS MEDIUM, MODIFIED

Product Code TM 1127

  • Description

    Semisolid Rappaport Vassiliadis Medium, modified is prepared as per the formulation described by DeSmedt et al, for the detection of motile Salmonella species from food and environmental specimens. This medium detects more Salmonella positive samples than the routinely used enrichment procedures. The working of medium is based on the ability of Salmonella species to migrate in the selective medium by competing with the other motile organisms, thus producing opaque halos of growth. This medium can be used in combination with direct culture and Selenite F Broth enrichment for isolation of Salmonella species from faeces and subculturing on XLD Agar or Mannitol Lysine Agar results in higher recovery rates. This medium is not suitable for the detection of non-motile strains of Salmonella.
    Inoculate 3 drops (0.1 ml) of pre-enrichment culture (16-20 hours old) in separate spots on the air-dried medium surface. Incubate the plates in an upright position at 42?C for upto 24 hours. The motile bacteria will show a halo or zone of growth originating from inoculation spot. Salmonella species show straw coloured colonies. Sub-cultures can be carried out from the outside edge of the halo to confirm purity and for further biochemical and serological tests.

  • Principle

    for detection of motile Salmonella species from food, faeces and environmental specimens

  • Microorganism

    • Salmonella Enteritidis
    • Salmonella typhi
    • Salmonella Typhimurium
  • Industry

    • Food & Beverages
    • Industrial & Lab Purposes
    • Water
  • Pack Size

    • 500 gm
  • Downloads

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