SCHAEDLER BROTH

Schaedler Broth was originally formulated by Schaedler et al and modified by Mata et al with composition changes. It serves as an excellent basal medium to which blood or other enrichments can be added to enhance the recovery of fastidious anaerobic organisms. Stalons et al found this medium to be most effective medium for the growth of obligately anaerobic bacteria in an atmosphere of 5% carbon dioxide, 10% hydrogen and 85% Nitrogen. It can also be used to determine antibiotics MIC levels of anaerobic organisms. Fass et al used tube method for antibiotic MIC determination. Schaedler broth is highly nutritious medium due to tryptone, proteose peptone, soya peptone and yeast extract. Sodium Polyanethole Sulphonate (SPS) which is an anticoagulant in culture bottles promotes optimal recovery of organisms from blood. It acts to inhibit phagocytosis and to neutralize the antibacterial activity of fresh blood components

for cultivation of wide variety of microorganisms particularly from anaerobic blood cultures