For cultivation of yeasts, molds and aciduric microorganisms.
SABOURAUD DEXTROSE AGAR (SDA) is a modification of Dextrose Agar originally designed by ‘Raymond Sabouraud’. SDA used for the isolation of saprophytic and pathogenic fungi from a variety of sources containing large numbers of other fungi or bacteria. The high concentration of Dextrose is included as an energy source. The acidic pH (5.6) of this medium promotes the growth, formation of (sporangia and condia) as well as the formation of yeasts and molds. Addition of chloramphenicol supplement or antibiotics increases the selectivity of medium which inhibits many species of bacteria. Characteristics features of fungi and molds, such as sporing structures and pigmentation are well developed on this medium. SDA contains Mycological peptone provide nitrogen and vitamin source required for organism growth. Due to its low pH this medium is very sensitive to overheating which will soften the agar and caramelize the carbohydrate. Agar is the solidifying agent.