RAPPAPORT VASSILIADIS SOYABEAN MEAL BROTH (RVSM)

Product Code TM 1070

  • Description

    Rappaport Vassiliadis Soyabean Meal Broth (RVSM) is modification of the Rappaport Vassiliadis Enrichment Broth, revised by van Schothorst. This medium is recommended as the selective enrichment medium for isolation of Salmonella. van Schothorst modified the original formula by addition of dipotassium hydrogen phosphate to buffer the medium and addition of anhydrous magnesium chloride to enhance the reliability of enrichment broth. Peterz et al have also emphasized the importance of the concentration of magnesium chloride in the final medium.
    The test specimen is added to Buffered Peptone Water and incubated at 35?C for 16 – 20 hours. This pre-enriched peptone water culture is inoculated into RVSM Broth and incubated at 42 ? 1?C for 24 – 48 hours and further subcultured on Brilliant Green Agar. For faecal specimens, no pre-enrichment is needed. Add 1 or 2 loopful of liquid faeces (or an emulsion of faeces in saline) to 10 ml of RVSM Broth pre-warmed to 42?C. Incubate at 42 ?1?C for 24 hours and streak on to a selective agar.

  • Principle

    for enrichment and isolation of Salmonellae

  • Microorganism

    • Escherichia coli
    • Salmonella Paratyphi B
    • Salmonella typhi
    • Salmonella Typhimurium
  • Industry

    • Clinical Diagnostics
    • Food & Beverages
    • Water
  • Pack Size

    • 100 gm
    • 500 gm
  • Downloads

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