for cultivation and enumeration of yeasts and molds.
POTATO DEXTOSE BROTH (VEG.) is used for the isolation and enumeration and culturing of yeast and molds from samples. It can also be used in the identification of fungi and yeasts in parallel with their cellular morphology or in methods of micro cultivation in slides. Dextrose and Potato infusion (BEEVER and BOLLARD 1970) promote the growth of yeasts and moulds while the low pH value partially inhibits the growth of the accompanying bacterial flora. If the medium is to be used for fungal counts and enumeration of yeasts and mold, the pH should be adjusted to approximately 3.5 to inhibit bacterial count. By the addition of sterilized 10% tartaric acid solution to 1000ml of sterilized medium to obtain a pH of 3.5. Fungi grow on this medium to develop typical morphology. This general-purpose medium can be supplemented with acid or antibiotics to inhibit bacterial growth.