For cultivation of phosphate solubilizing microorganisms in commercial production of Biofertilizer. Pikovskaya’s Broth is a modification of Pikovskayas agar medium originally modified by Sundara Rao and Sinha for culturing phosphate solubilizing microorganisms. Both inorganic and organic phosphates exist in soil. Many naturally occurring soil fungi and bacteria are phosphate solubilizers and they play an important role in maintaining phosphorus balance of crop plants. This fact is exploited in culturing phosphate solubilzers which are able to solubilize bound phosphates. Phosphate as calcium phosphate is present in the medium. Dextrose acts as energy source. Different salts and yeast extract supports the growth of organisms.