For selective enrichment of Y. enterocolitica. Dehydrated powder, store in a dry place, in tightly-sealed containers at 24°C and protect from direct Sunlight.
PEPTONE SORBITOL BROTH is used for selective enrichment of Y. enterocolitica. . Swine are recognized as major reservoirs of Y. enterocolitica in nature. Outbreaks of gastroenteritis associated with Yersinia enterocolitica are increasing. Contaminated food products such as pork, beef, and raw and processed milk have been identified as sources of infection. Enzymatic Digest of Casein provides nitrogen, vitamins, minerals and amino acids essential for growth. Sodium chloride supplies essential electrolytes for transport and osmotic balance. Potassium phosphates act as a buffer system. The pH of the medium is slightly alkaline as Yersinia species are tolerant to dilute alkali. Sorbitol is the fermentable carbohydrate providing carbon and energy. Bile salt inhibit majority of the gram-positive organisms.Inoculate 25 grams of sample into 225 ml of PEPTONE SORBITOL BROTH (TM 1844). Incubate at 10°C for 10 days. After incubation, spread 0.1 ml onto YERSINIA SELECTIVE AGAR BASE (TM 501). Presumptive Yersinia colonies are confirmed by appropriate biochemical test.